为探究中药复方多糖对鸡新城疫抗体含量、T 淋巴细胞转化率及 IgG、IgM 含量的影响，本研究将 200 羽黄麻肉鸡
分成高、中、低剂量组和空白对照组，每组 50 羽，于 7、28 日龄进行新城疫疫苗免疫；各试验组鸡于 8 日龄分别肌肉注射
不同剂量纯化中药复方多糖，连续 7 d；采集 21、42、56、70 日龄鸡外周血，ELISA 法检测血清中新城疫抗体、IgG 和 IgM 含
量，MTT 法测定 T 淋巴细胞转化率。结果显示：与空白对照组相比，各试验组鸡新城疫抗体含量、T 淋巴细胞转化率及
IgG、IgM 的含量均有显著的提高(P<0.05)；中剂量组免疫增强效果显著高于高剂量组和低剂量组 (P<0.05)，高剂量组免疫
增强效果高于低剂量组。由此可见，中剂量多糖免疫增强作用优于高剂量和低剂量，为增强免疫作用的最佳浓度。
Abstract
To explore the effects of traditional Chinese medicine compound polysaccharide on Newcastle Disease (ND) antibody
content, T lymphocyte transformation rate and IgG, IgM content. 200 HuangMa chickens randomly divided into high does group,
middle does group, low does group and control group. Each group was vaccinated with NDV at age of 7 and 28 days old
respectively. Each experimental group intramuscular injection of three different purified polysaccharide traditional Chinese
medicine at 8- day- old, for 7 consecutive days. Detected 21, 42, 56, 70- day- old chicken serums Newcastle Disease antibody
content and IgG, IgM content by ELISA, detected T lymphocyte transformation rate by MTT. The results showed that the
experimental groups Newcastle Disease antibody content, T lymphocyte transformation rate and IgG, IgM content was significantly
improved, which compared with the control group (P<0.05). The middle dose group was significantly higher than that of high
dose group and low dose group (P<0.05), and high dose group higher than the low dose group. It is showed that the best
immune enhancing effect is the median concentrations of polysaccharide
关键词
复方多糖 /
鸡 /
新城疫 /
免疫球蛋白 /
淋巴细胞转化率
{{custom_keyword}} /
Key words
polysaccharide compound /
chicken /
Newcastle Disease /
immunoglobulin /
T lymphocyte transformation rate
{{custom_keyword}} /
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] 王仲挺.小规模鸡场新城疫免疫失败的原因及对策[J].中
国畜禽种业，2015，11(2):143-145.
Wang Z T.Causes and countermeasures for small-scale
farms Newcastle immune failure [J].Chinese Livestock and
Poultry Seed Industry，2015，11(2):143-145.
[2] 朱晓磊，祁凤华，黄金涛，陈宏.百里香精油对麻花鸡生长
性能及免疫器官指数的影响[J].石河子大学学报(自然科
学版)，2013，31(1):43-47.
Zhu X L,.Qi F H,Huang J T,et al.Effect of dietary thyme
essential oil on growth performance and immune organ
indices in Mahua Broiler Chickens [J].Journal of Shihezi
University(Natural Science)，2013，31(1):43-47.
[3] 祁凤华，扇玉斌，周立强，等.鸡源性嗜酸乳杆菌对肉鸡小
肠黏膜免疫相关细胞的影响[J].石河子大学学报(自然科
学版)，2015，31(1):50-53.
Qi F H,Shan Y B,Zhou L Q,et al.Effects of lactobacillus
acidophilus isolate from brioler on mucosal immunityassociated cells in small intestine of Broiler[J].Journal of Shihezi
University(Natural Science)，2015，31(1):50-53.
[4] Fan Y，Lu Y，Wang D，et al.Effect of epimedium polysaccharide-propolis flavone immunopotentiator on immunosuppression induced by cyclophosphamide in chickens[J].Cellular Immunology，2013，281(1):37-43.
[5] Lee J H，Choi Y H，Kang H S，et al.An aqueous extract
of platycodi radix inhibits LPS-induced NF-kappa B
nucleartranslocation in human cultured airway epithelial
cells[J].Inter National Journal of Molecular Medicine，2004，
13(6)：843-847.
[6] Jia Rui，Cao Liping，Xu Pao.In vitro and in vivo hepatoprotective and antioxidant effects of Astragalus polysaccharides against carbon tetrachloride-induced hepatocyte damage in common carp (Cyprinus carpio) [J].Fish Physiology
and Biochemistry，2012，38(6):871-881.
[7] 王宏军. 南五味子多糖提取及其对雏鸡免疫活性的研究
[D].吉林：吉林大学，2011.
[8] Nikbakht-Brujeni G，Tajbakhsh H，Pooyanmehr M，et al.In
vitro immunomodulatory effects of Astragalus verus Olivier.
(black milkvetch): an immunological tapestry in Kurdish
ethnomedicine[J].Comp Clin Pathol，2011，11:176-187.
[9] Qingfang YANG，Kanglin YANG.Effects of three kinds of
chinese medicine polysaccharides on the immune effect of
Newcastle Disease vaccine[J].Agricultural Science & Technology，2015，16(10):2283-2285.
[10] 封海波. 川牛膝多糖对小鼠免疫反应的影响及机理研究
[D].雅安：四川农业大学，2013.
[11] 李赞阳，刘红，罗燕，等.不同组分、不同纯度中药复方多
糖对小鼠免疫活性影响的研究[J].中国畜牧兽医，2012,39
(9):181-184.
Li Z Y,Liu H,Luo Y,et al.Research on Chinese Medicinal
Compound Polysaccharide’s influence on mice immunocompetence with different components and different purities
[J].China Animal Husbandry & Veterinary Medicine，2012,
39(9):181-184.
[12] 李赞阳，刘红，李炳奇，孙萍，谷新利.AB-8 大孔树脂吸附、
解吸中药复方多糖的研究 [J]. 黑龙江畜牧兽医，2011(19):
115-117.
Li Z Y,Liu H,Li B Q,et al.Research of AB-8 macroporous
rsin adsorption and desorption chinese medicine compound
Polysaccharides[J].Heilongjiang Animal Science and Veterinary Medicine，2011(19):115-117.
[13] 李赞阳，刘红，罗燕，等.不同组分及不同纯度中药复方多
糖对小鼠免疫功能的影响[J].西北农业学报，2012，21(9):
15-18.
Li Z Y,Liu H,Luo Y,et al.Influence of Chinese Medicinal
Compound Polysaccharide with different components and
different purities on mice immune function[J].Acta Agriculturae Boreali-accidentalis Sinica，2012，21(9):15-18.
[14] 乔海博，谷新利，朱晓庆，等.复方中药多糖不同给药途径
对鸡淋巴细胞转化率的影响 [J]. 江苏农业科学，2013，41
(10):183-185.
Qiao H B,Gu X L,Zhu X Q,et al.Effect of compound Chinese medicine polysaccharide different routes of administration for the chicken lymphocyte transformation[J].Jiangsu
Agricultural Science，2013，41(10):183-185.
[15] 刘蓉蓉，范强，高珍珍，等.香菇多糖对雏鸡新城疫疫苗免
疫的增强作用[J].畜牧与兽医，2014，46(10):86-89.
Liu R R,Fan Q,Gao Z Z,et al.The Enhancement effect of
polysaccharide from letinous edodes on immune function
of New Castle Disease vaccine in chickens[J].Animal Husbandry & Veterinary Medicine，2014，46(10):86-89.
[16] 乔海博，谷新利，朱晓庆，等.复方中药多糖对鸡新城疫疫苗
免疫效果的影响[J].石河子大学学报(自然科学版)，2013，30
(6):700-703.
Qiao H B,Gu X L,Zhu X Q,et al.Effect of compound polysaccharide on immune efficacy of Newcastle Disease vaccine in chicken[J].Jiangsu Agricultural Science，2013，30(6):
700-703.
[17] 陈家磊，刘师旗，冯君，等.车前草多糖对雏鸡新城疫疫苗
免疫效果的影响[J].中国兽医杂志，2015，51(10):50-52.
Chen J L,Liu S Q,Feng J,et al.Effects of plantain polysaccharide on Newcastle Disease vaccine in chicken[J].Chinese Journal of Veterinary Medicine，2015，51(10):50-52.
[18] Wang D Y,Hu Y L,Sun J L,et al.Comparative study on
adjuvanticity of compound Chinese herbal medicinal ingredients[J].Vaccine，2005.23(28):3704-3708.
[19] 王佳丽，徐聪.复方中草药对肉鸡生长性能及免疫功能的
影响[J].饲料研究，2012(1):24-26.
Wang J L,Xu C.Effect of compound Chinese Herbal Medicine on growth performance and immune function[J].Feed
Research，2012(1):24-26.
[20] 梁英，姜宁，何雯娟，等.黄芩多糖对肉仔鸡生长性能和免
疫功能的影响[J].动物营养学报，2010，22(4):1031-1036.
Liang Y,Jiang N,He W J,et al.Effect of Scutellaria polysaccharide on growth performance and immune function [J].
Chinese Journal of Animal Nutrition，2010，22(4):1031-1036.
[21] 王思芦，汪开毓，赵玲，等.鸡枞菌多糖对免疫抑制小鼠免
疫功能的影响[J].中国药理学通报 2013，29(1):59-63.
Wang S L,Wang K Y,Zhao L,et al.Immunolore effect of
polysaccharide from termitomy-ces albuminosus (Berk.)
Heim on immumosuppressive mice[J].Chinese Pharmacological
Bulletin，2013，29(1)：59-63.
[22] 商云霞，朱晓庆，谷新利，等.一定纯度中药复方多糖对鸡
细胞免疫和体液免疫的影响[J].中国畜牧兽医，2015，42(4):
943-949.
Shang Y X,Zhu X Q,Gu X L,et al.Effects of certain purified chinese herbal medicinal compound polysaccharide
on celluar immunity and humoral immunity in chicken[J].
China Animal Husbandry & Veterinary Medicine，2015，
42(4):943-949.
[23] 章世元，徐春燕，董晓芳，等.苜蓿多糖和黄芪多糖对肉仔
鸡淋巴细胞增殖的影响 [J]. 动物营养学报，2010，22(3):
670-674.
Zhang S Y,Xu C Y,Dong X F,et al.Alfalfa polysaccharides
and astragalus polysaccharides on lymphocyte proliferation
of broilers[J].Chinese Journal of Animal Nutrition，2010，22
(3):670-674.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
国家自然科学基金项目(31460673)
{{custom_fund}}
PDF(231 KB)
